BRUKER APEX II MASS SPECTROMETER FULL

When employing selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) modes, both Q 1 and Q 3 are set at a specific mass, allowing only a distinct fragment ion from a certain precursor ion to be detected. Similar to the precursor ion scan, this method is useful in the selective identification of closely related compounds in a mixture. This allows the selective recognition of all ions which, by fragmentation in q 2, lead to the loss of a given neutral fragment (e.g., H 2O, NH 3). In the neutral loss scan method both Q 1 and Q 3 are scanned together, but with a constant mass offset. This method is selective for ions having a particular functional group (e.g., a phenyl group) released by the fragmentation in q 2. When utilizing a precursor scan, a certain product ion is selected in Q 3, and the precursor masses are scanned in Q 1. This method is commonly performed to identify transitions used for quantification by tandem MS. The structure of the original ion can be deduced from the ion fragmentation information.

The third quadrupole Q 3 is then set to scan the entire m/z range, giving information on the sizes of the fragments made. In the product scan, the first quadrupole Q 1 is set to select an ion of a known mass, which is fragmented in q 2. The arrangement of the TQMS allows for four different scan types to be performed: a precursor ion scan, neutral loss scan, product ion scan, and selected reaction monitoring. Settings associated with selecting m/z values in both mass filters of a triple quadrupole mass analyzer The triple quadrupole allows the study of low-energy low-molecule reactions, which is useful when small molecules are being analyzed. Īlso, when operated in the selected reaction monitoring mode, the TQMS has superior detection sensitivity as well as quantification. However, the triple quadrupole has the advantage of being cheaper, easy to operate and highly efficient. Sector instruments tend to surpass the TQMS in mass resolution and mass range. The TQMS follows the tandem-in-space arrangement, due to ionization, primary mass selection, collision induced dissociation (CID), mass analysis of fragments produced during CID, and detection occurring in separate segments of the instrument. Unlike traditional MS techniques, MS/MS techniques allow for mass analysis to occur in a sequential manner in different regions of the instruments. In some instruments, the normal quadrupole collision cell has been replaced by hexapole or octopole collision cells which improve efficiency. The RF potential associated with the collision cell (q) allows all ions that were selected for to pass through it. Both Q1 and Q3 are controlled by direct current (dc) and radio-frequency (rf) potentials, while the collision cell, q, is only subjected to RF potential. Each of the two mass filters (Q1 and Q3) contains four parallel, cylindrical metal rods. Principle of operation Įssentially the triple quadrupole mass spectrometer operates under the same principle as the single quadrupole mass analyzer. It was later found that the triple-quadrupole mass spectrometer could be utilized to study organic ions and molecules, thus expanding its capabilities as a tandem MS/MS technique. In the years following, the first commercial triple-quadrupole mass spectrometer was developed at Michigan State University by Enke and Yost in the late 1970s. Enke and his then graduate student Richard Yost, Morrison's linear arrangement of the three quadrupoles probed the construction of the first triple-quadrupole mass spectrometer.

Morrison of LaTrobe University, Australia for the purpose of studying the photodissociation of gas-phase ions.

The exact mass measurements are generally accurate to within 1-2 ppm on positive ions for masses up to 1500 Da.The arrangement of three quadrupoles was first developed by J.D.

BRUKER APEX II MASS SPECTROMETER FULL

It is capable of doing both mid resolution, full mass range scans, as well as high resolution, exact mass measurements. Simonetta Fornarini ( FT-ICR mass spectrometer is capable of analyzing organic/inorganic molecules using EI, CI or ESI. Fornarini and Crestoni (Ion Trap Bruker Esquire 3000+ mass spectrometer and API 2000 Applied Biosystems triple quadrupole mass spectrometer). Direct access to mass spectrometry training, expertise and instrumentation is available on the other mass spectrometers run by the research group lead by profs. The instrument is not available for student hands-on use. Prospective users can also send an email for inquiries. A customized plan will be set to meet the research requests. FT-ICR mass spectrometry lab - Principles of access to the serviceĮxternal users are invited to submit a detailed description of their project.

Bruker apex ii mass spectrometer